Thea Tsulukiani was born in Tbilisi, on January 21, 1975.
In 1992 Thea Tsulukiani graduated from the Collège-lycée Ampère in Lyon, France, with undergraduate Bachelor degree.
In 1992 she entered Tbilisi State University, faculty of International Law and International Relations, which she successfully graduated from in 1997. At the same time, she studied at the Academy of Diplomacy of Ministry of Foreign Affairs, which she graduated in 1998.
From 1997 to 1998, she worked for the Foreign Policy Research and Analysis Center at Ministry of Foreign Affairs of Georgia.
In 1998, she successfully passed the competitive examination for the National School of Administration - École Nationale d'Administration (ENA), which is under guidance of the French Prime Minister and trains various high-rank officials and administrative justice judges. During her studies at ENA, she worked for the Cabinet of Prefect of the Department of Sarthe, (Western France) on the issues with regard to defense public order in the problematic areas of the city and those of coordination of the activities of government bodies at a local level.
In 2000, Thea Tsulukiani graduated from ENA with a Masters degree in Public Administration (MPA).
Between 2000 and 2010, she worked as a permanent civil servant of the Council of Europe and was employed by the European Court of Human Rights in Strasbourg (ECHR) on the position of a lawyer. Her duties were to examine and draft decisions and judgments on the applications brought against Georgia and partly Russia. In 2005, she was promoted to the class rank of Senior Lawyer of the Court. From 2006, she was a member of the Committee working on the Rules of the Court; from 2009 - a rapporteur on the cases examined by single-judge compositions.
On February 24, 2010, Thea Tsulukiani resigned from her position at the ECHR and returned to Georgia.
On May 15, 2010, she joined the political party of Free Democrats as the party chairperson's adviser on human rights, and was later appointed as a deputy chairperson of the party. At the same time, she was in charge of women's rights issues within the party.
On October 1, 2012, Thea Tsulukiani took part in the parliamentary election as a candidate in a single-mandate constituency upon nomination of the political coalition "Bidzina Ivanishvili - Georgian Dream Coalition".
She obtained 72% of the vote in the Nadzaledevi District of Tbilisi and became a Member of Parliament.
On October 25, 2012, she was appointed as Minister of Justice of Georgia.